Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

40 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Molecular Changes That Ooccur to Repair Radiation Damage and Gene Transcripts That May be Involved in Establishing Disease in Mice Post Irradiation

ABSTRACT: The protozoan parasite Trypanosoma evansi is responsible for causing Surra in a variety of mammalian hosts over a wide geographical area. In order to identify which genes and processes are required to establish disease in mice, parasites were irradiated over a range using a Cobalt60 gamma source. A custom Trypanosome spp. array that covers the genomes of three trypanosome species, T. brucei, T. evansi and T. congolense was designed by Affymetrix with an average of 9300 whole gene transcripts from all three species were targeted. Irradiation differentially affected the abundance of gene transcripts in a dose-dependent trend. We present these genes as necessary for repair from irradiation damage, and essential for disease establishment in mice post irradiation.

ORGANISM(S): Trypanosoma evansi

SUBMITTER: Richard Thiga Kangethe

PROVIDER: E-MTAB-11705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Time series RNA-seq of temperature-entrained and light-entrained bloodstream and procyclic form Trypanosoma brucei cultures

Project description:To address whether T. brucei has a circadian clock we probed its transcriptome by RNA-seq, searching for transcripts oscillating with a 24 hr period. For this we entrained/ synchronized parasites in vitro for three days using temperature and light as environmental stimuli and then collected parasite RNA every four hours for two consecutive days. Parasite RNA was subjected to RNA-seq analysis. We performed this protocol on two stages of the T. brucei life cycle (bloodstream and insect procyclic forms).

2017-02-10 | E-MTAB-4952 | biostudies-arrayexpress

Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Gene Transcripts Involved in Establishing Disease in Mice Post Irradiation

Project description:Transcriptome analysis of irradiated T evansi parasites The protozoan parasite Trypanosoma evansi is responsible for causing Surra in a variety of mammalian hosts over a wide geographical area. In order to identify which genes and processes are required to establish disease in mice, parasites were irradiated over a range using a Cobalt60 gamma source. A custom Trypanosome spp. array that covers the genomes of three trypanosome species, T. brucei, T. evansi and T. congolense was designed by Affymetrix with an average of 9300 whole gene transcripts from all three species were targeted. Irradiation differentially affected the abundance of gene transcripts in a dose-dependent trend. We present these genes as necessary for repair from irradiation damage, and essential for disease establishment in mice post irradiation.

2023-05-06 | GSE202334 | GEO

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

Project description:Here we report the proteome content of exosomes released by epimastigote and bloodstream trypomastigotes forms of T. cruzi, the aetiological agent of Chagas disease. The exosomes were isolated using the ultracentrifugation method and further processed for LC-MS/MS. The results showed differential proteomic profiles of this two forms with marked differences in surface molecules cargoes. Noteworthy, the trans-sialidase (TS) surface proteins, a family of polymorphic virulence factors were differentially expressed between insect and human stages of T. cruzi highlighting the different surface nature of this two parasite stages.

2021-09-08 | PXD013901 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013880 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013876 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013882 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013875 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013878 | Pride

H3K9me3 Cut&Tag on hematopoietic stem cells sorted from mice 1 month after they were irradiated (2Gy total body irradiation = IR) or not (non irradiated = NIR) and with or without prior TNFa treatment (IR_TNF)

Project description:Analysis of the RNA-seq data performed in IR vs NIR hematopoietic stem cells show the loss of the TNF_via_NFKB signature. We showed that the loss of this signature could be associated with H3K9me3 loss at specific retrotransposable elements . To validate this association, we tested if TNFa treatment before irradiation was able to prevent IR-effect on H3K9me3 loss at retrotransposable elements. For this purpose, we treated mice with TNFa 1h before irradiation (IR_TNF) and performed H3K9me3 cut&tag experiments on hematopoietic stem cells 1 month after irradiation and compared them to hematopoietic stem cells sorted from non irradiated mice (NIR) and from non-treated irradiated mice (IR).

2022-06-24 | E-MTAB-11864 | biostudies-arrayexpress

Identification of CARP3-interacting proteins in Trypanosoma brucei bloodstream forms and procyclic forms

Project description:How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. The trypanosome-specific cAMP response protein 3 (CARP3) shows developmentally regulated localization at the flagellar tip membrane, where it is essential for parasite swarming and colonization of the tsetse fly insect vector. This project describes identification of CARP3-YFP interacting proteins by GFP trap pull down followed by mass spectrometry in the bloodstream stage as well as in the procyclic stage of Trypanosoma brucei.

2022-08-31 | PXD025398 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data