Proteomics

Dataset Information

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Identification of CARP3-interacting proteins in Trypanosoma brucei bloodstream forms and procyclic forms

ABSTRACT: How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. The trypanosome-specific cAMP response protein 3 (CARP3) shows developmentally regulated localization at the flagellar tip membrane, where it is essential for parasite swarming and colonization of the tsetse fly insect vector. This project describes identification of CARP3-YFP interacting proteins by GFP trap pull down followed by mass spectrometry in the bloodstream stage as well as in the procyclic stage of Trypanosoma brucei.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trypanosoma Brucei Brucei

SUBMITTER: Ignasi Forne  

LAB HEAD: Boshart, Michael

PROVIDER: PXD025398 | Pride | 2022-08-31

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Ref2138_1.6.2.2_MB_20190531.sps Other
Ref2138_MB_BSF_WT1_20190529.raw Raw
Ref2138_MB_BSF_WT2_20190529.raw Raw
Ref2138_MB_BSF_YFP1_20190529.raw Raw
Ref2138_MB_BSF_YFP2_20190529.raw Raw
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Publications

Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonizat  ...[more]

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