Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of adult Heliconius melpomene rosina male and female chemosensory tissues: antennae, labial palps and proboscis, and legs

ABSTRACT: The purpose of this experiment was to compare RNA-seq profiles of adult male and female butterfly chemosensory tissues to identify tissue- and sex-specific differences in gustatory and olfactory gene expression. Three biological replicates per sex were produced from individual Heliconius melpomene rosina butterflies. For the antennal libraries, both antennae were used, for the labial palps + proboscis libraries both labial palps and each proboscis was used, and for the leg libraries all six legs were used.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Heliconius melpomene rosina

SUBMITTER: Adriana Briscoe

PROVIDER: E-MTAB-1500 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Female behaviour drives expression and evolution of gustatory receptors in butterflies.

Briscoe Adriana D AD Macias-Muñoz Aide A Kozak Krzysztof M KM Walters James R JR Yuan Furong F Jamie Gabriel A GA Martin Simon H SH Dasmahapatra Kanchon K KK Ferguson Laura C LC Mallet James J Jacquin-Joly Emmanuelle E Jiggins Chris D CD

PLoS genetics 20130711 7

Secondary plant compounds are strong deterrents of insect oviposition and feeding, but may also be attractants for specialist herbivores. These insect-plant interactions are mediated by insect gustatory receptors (Grs) and olfactory receptors (Ors). An analysis of the reference genome of the butterfly Heliconius melpomene, which feeds on passion-flower vines (Passiflora spp.), together with whole-genome sequencing within the species and across the Heliconius phylogeny has permitted an unpreceden ...[more]

PMID: 23950722

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Project description:During mammalian pre-implantation embryonic development dramatic and orchestrated changes occur in gene transcription. The identification of the complete changes has not been possible until the development of the Next Generation Sequencing Technology. Here we report the first transcriptome dynamics of single matured bovine oocytes and all stages of pre-implantation embryos developed in vivo. Surprisingly, nearly half of the bovine genome, 11,488 to 12,729 genes involved in more than 100 pathways, is expressed in oocytes and early embryos. Despite the similarity in the total numbers of genes expressed across stages, the nature of the expressed genes is dramatically different. A total of 2,845 genes were differentially expressed among different stages, of which the largest change was observed between the 4- and 8-cell stages, demonstrating that the bovine embryonic genome activation occurs at this transition. Additionally, 774 genes were identified as only expressed/highly enriched in particular stages of development. Using weighted gene co-expression network analysis, we found 12 stage-specific modules of co-expressed genes that can be used to represent the corresponding stage of development. Furthermore, we identified conserved key members (or hub genes) of the bovine expressed gene networks. Their vast association with other embryonic genes suggests that they may have important regulatory roles in embryogenesis; yet, the majority of the hub genes are relatively unknown/under-studied in embryos. We also conducted the first embryonic expression profile comparison across three mammalian species, human, mouse and bovine, for which RNA-seq data are available. We found that the three species share more maternally deposited genes than embryonic genome activated genes. More importantly, there are more similarities in embryonic transcriptomes between bovine and humans than between humans and mice, demonstrating that bovine embryos are better models for human embryonic development. This study provides the first comprehensive examination for gene activities in bovine embryos and identified little-known potential master regulators of pre-implantation development. RNA-Seq profiles from single in vivo matured oocytes and in vivo developed embryos from the 2-cell to the blastocyst stages generated using Solid RNA-seq platform.

Dataset Information

RNA-seq of adult Heliconius melpomene rosina male and female chemosensory tissues: antennae, labial palps and proboscis, and legs

Publications

Female behaviour drives expression and evolution of gustatory receptors in butterflies.

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