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MLE HITS-CLIP in Drosophila S2 cells

ABSTRACT: HITS-CLIP was carried out on wild-type Schneider (S2) cells using specific MLE antibodies to identify binding sites for MLE in the Drosophila transcriptome

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Filipe Tavares-Cadete

PROVIDER: E-MTAB-1706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tandem stem-loops in roX RNAs act together to mediate X chromosome dosage compensation in Drosophila.

Ilik Ibrahim Avsar IA Quinn Jeffrey J JJ Georgiev Plamen P Tavares-Cadete Filipe F Maticzka Daniel D Toscano Sarah S Wan Yue Y Spitale Robert C RC Luscombe Nicholas N Backofen Rolf R Chang Howard Y HY Akhtar Asifa A

Molecular cell 20130701 2

Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long noncoding RNAs (lncRNAs) for transcriptional upregulation of the male X chromosome. Here, by using UV crosslinking followed by deep sequencing, we show that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo. These domains constitute the minimal RNA unit present in multi ...[more]

PMID: 23870142

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