Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Identification of carboxylesterase genes implicated in temephos resistance in the dengue vector Aedes aegypti

ABSTRACT: The transcriptome profilling of an Aedes aegypti strain from Thailand resistant to Permethrin and Temephos (Nakhon Sawan 2, NS2) was compared to a susceptible strain of Thailand: Phatthalung (P). A experimental loop design was used : NS2 larvae unexposed vs P, NS2 Exposed (larvae survivors of a temephos bioassay inducing 65% mortality) vs P and NS2 Exposed vs NS2 Unexposed.

ORGANISM(S): Aedes aegypti

SUBMITTER: Rodolphe Poupardin

PROVIDER: E-MTAB-1934 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of carboxylesterase genes implicated in temephos resistance in the dengue vector Aedes aegypti.

Poupardin Rodolphe R Srisukontarat Wannaporn W Yunta Cristina C Ranson Hilary H

PLoS neglected tropical diseases 20140320 3

<h4>Background</h4>Thailand is currently experiencing one of its worst dengue outbreaks in decades. As in most countries where this disease is endemic, dengue control in Thailand is largely reliant on the use of insecticides targeting both immature and adult stages of the Aedes mosquito, with the organophosphate insecticide, temephos, being the insecticide of choice for attacking the mosquito larvae. Resistance to temephos was first detected in Aedes aegypti larvae in Thailand approximately 25 y ...[more]

PMID: 24651719

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Project description:Cotton seeds (Gossypium hirsutum cv. CCRI12) were grown in a growth chamber under 29/25Â°C temperature and a 16:8 h light:dark cycle, and water was added every two days. All plants were used in experiments at the 6-7 fully expanded true leaf stage, which occurred 5-6 weeks after sowing. Cotton bollworm (CBW; Helicoverpa armigera) larvae were reared on an artificial diet and maintained at 27 Â± 2Â°C, 75 Â± 10% relative humidity, and 14:10 h light:dark in the laboratory. For insect treatment, seven H. armigera larvae (third instars) were placed on a group of three plants, which were kept within plastic bags (30 Ã? 40 cm), until time of harvest, with samples for each time point maintained separately. Undamaged plants maintained under the same conditions were used as controls. Cotton leaves from control plants and plants exposed to H. armigera were harvested at 6 h, 12 h, 24 h, and 48 h after onset of herbivory. For each treatment group and time point, cotton leaves were harvested from the three plants per treatment group and flash frozen in liquid nitrogen. For each time point, three replicate treatments and controls were performed. For insect treatment, seven H. armigera larvae (third instars) were placed on a group of three plants, which were kept within plastic bags (30 Ã? 40 cm), until time of harvest, with samples for each time point maintained separately. Undamaged plants maintained under the same conditions were used as controls. Cotton leaves from control plants and plants exposed to H. armigera were harvested at 6 h, 12 h, 24 h, and 48 h after onset of herbivory. For each treatment group and time point, cotton leaves were harvested from the three plants per treatment group and flash frozen in liquid nitrogen. For each time point, three replicate treatments and controls were performed. In this study we present dynamic transcriptome analysis and volatile profiling of cotton plants fed upon by larvae of a leaf-chewing herbivore CBW. Plant transcriptomic changes induced by CBW were analyzed using Affymetrixâ??s Cotton GeneChips. Samples from a time course of six hour to 48 hours following onset of CBW feeding were analyzed to identify target genes and key pathways involved in the activation of herbivory-induced indirect defense and to explore genetic basis of such defense. In addition, we monitored the accumulation of VOCs, which represent changes in cotton plant phenotype, following CBW infestation.

Dataset Information

Identification of carboxylesterase genes implicated in temephos resistance in the dengue vector Aedes aegypti

Publications

Identification of carboxylesterase genes implicated in temephos resistance in the dengue vector Aedes aegypti.

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