Project description:Wild-type A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07M-^V1.08) under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22C. Cold treatment: The 3-week-old plants were transferred from 22C to 4C and were grown for 1 day.
Project description:Wild-type A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07M-^V1.08) under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22C. Dehydration treatment: The 3-week-old plants were grown for 6 days without watering.
Project description:Wild Type and OsTZF5-OX plants were grown in soil in isolation green house for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (1000 umol photons/m2/s) at 28C (day) and 25C (night).
Project description:Gene expression profiles in transgenic rice plants that overexpressed OsNAC5, driven by the maize ubiquitin promoter (UBIpro::OsNAC5).
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 M-1 10 ?mol photons/m2/s) at 28M-0C (day) and 25M-0C (night). For cold treatment, two-week-old plants were transferred from 28M-0C to 10M-0C and incubated for 1 day.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 ± 10 ?mol photons/m2/s) at 28°C (day) and 25°C (night). For dehydration treatment, two-week-old plants were incubated for 3 days without watering. The soil moisture content was 15.6% on day 3 of dehydration.
Project description:Vector control and OsTZF1-OX rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (1000 umol photons/m2/s) at 28C (day) and 25C (night). For NaCl treatment, two-week-old plants were transferred to 250 mM NaCl solution and incubated for 2 days under the above conditions.
Project description:Total RNA was isolated with RNAiso regent (Takara) from node portions at a heading stage of transgenic rice (Oryza sativa L. cv. Nipponbare) plants overexpressing OsPIF1 and vector control plants grown in green house at 28 C (day) and 25 C (night) under natural light conditions supplemented with metal halide lamps to provide 14-h daylength.
Project description:Effect of single or double knock-out mutation of subclass III SnRK2 protein kinases in Arabidopsis after 6 h of treatment with dehydration stress.