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Analysis of CITED4 shRNA mediated knockdown in the CRC cell line SW480

ABSTRACT: Empty vector, control shRNA and CITED4 shRNA permanent cell lines were generated by lentiviral transduction of the CRC cell line SW480 with either the empty vector (plko1), a control shRNA containing vector or a CITED4 shRNA containing vector and selected with puromycin in order to prepare permanent cell lines. For microarray analysis, the three cell lines were plated at a density of 1 x 10E5 cell in 12 well plates in 1 ml RPMI plus 10% FCS and penicillin/ streptomycin. The respective cell lines were harvested at day 1, 3 and 5 for RNA preparation and microarray analysis using Agilent human 4 x 44k expression microarrays. Every time point (cy3 labeled) was hybridzed together with Stratagene Human Total RNA standard as a control (cy5). Comparisons were made between the CITED4 shRNA permanent cell line and either the control shRNA or empty vector cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Rogers

PROVIDER: E-MTAB-2487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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