Unknown,Transcriptomics,Genomics,Proteomics

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SiRIP140 and gene expression effects in E2-treated MCF7 cells


ABSTRACT: This experiment is designed to assess the role of RIP140 in estrogen receptor-dependent gene expression in MCF7 luminal breast cancer cells. MCF7 cells were transfected with an siRNA to target RIP140 or siControl, and subsequently depleted from hormones for three days. Thereafter, cells were treated for 6 hours with 10nM Estradiol and RNA was isolated and further processed for expression analyses.

ORGANISM(S): Homo sapiens

SUBMITTER: Gordon Brown 

PROVIDER: E-MTAB-2577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Complex formation and function of estrogen receptor α in transcription requires RIP140.

Rosell Meritxell M   Nevedomskaya Ekaterina E   Stelloo Suzan S   Nautiyal Jaya J   Poliandri Ariel A   Steel Jennifer H JH   Wessels Lodewyk F A LF   Carroll Jason S JS   Parker Malcolm G MG   Zwart Wilbert W  

Cancer research 20140821 19


RIP140 is a transcriptional coregulator involved in energy homeostasis, ovulation, and mammary gland development. Although conclusive evidence is lacking, reports have implicated a role for RIP140 in breast cancer. Here, we explored the mechanistic role of RIP140 in breast cancer and its involvement in estrogen receptor α (ERα) transcriptional regulation of gene expression. Using ChIP-seq analysis, we demonstrate that RIP140 shares more than 80% of its binding sites with ERα, colocalizing with i  ...[more]

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