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Transcription profiling of the wild type and the deletion mutants of the bldM and whiI developmental genes of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth

ABSTRACT: BldM and WhiI are both orphan, atypical response regulators found in Streptomyces species. They have complex developmental roles in aerial mycelium formation and sporulation in these bacteria. The aim of this transcription profiling experiment was to see how the deletion of these genes affects genome wide transcript levels compared to the wild type.

ORGANISM(S): Streptomyces venezuelae

SUBMITTER: Govind Chandra

PROVIDER: E-MTAB-2716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712.

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Transcriptional profiling of the developmental gene whiH deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

Project description:WhiH is a transcription factor found in Streptomyces species. It has a role in sporulation in these bacteria. Although mutants in whiH are able to form aerial mycelia, these fail to differentiate into spores. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiH deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

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Project description:The sporulating, filamentous soil bacterium Streptomyces venezuelae ATCC 10712 differentiates under submerged and surface growth conditions. In order to lay a solid foundation for the study of development-associated division for this organism, a congenic set of mutants was isolated, individually deleted for a gene encoding either a cytoplasmic (i.e. ftsZ) or core inner membrane (i.e. divIC, ftsL, ftsI, ftsQ, ftsW) component of the divisome. While ftsZ mutants are completely blocked for division, single mutants in the other core divisome genes resulted in partial, yet similar, blocks in sporulation septum formation. Double and triple mutants for core divisome membrane components displayed phenotypes that were similar to those of the single mutants, demonstrating that the phenotypes were not synergistic. Division in this organism is still partially functional without multiple core divisome proteins, suggesting that perhaps other unknown lineage-specific proteins perform redundant functions. In addition, by isolating an ftsZ2p mutant with an altered -10 region, the conserved developmentally controlled promoter was also shown to be required for sporulation-associated division. Finally, microscopic observation of FtsZ-YFP dynamics in the different mutant backgrounds led to the conclusion that the initial assembly of regular Z rings does not per se require the tested divisome membrane proteins, but the stability of Z rings is dependent on the divisome membrane components tested. The observation is consistent with the interpretation that Z ring instability likely results from and further contributes to the observed defects in sporulation septation in mutants lacking core divisome proteins.

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