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Transcription profiling by array of E. coli steady-state cultures grown at different aerobiosis levels

ABSTRACT: E. coli steady-state cultures were grown at different aerobiosis levels; transcriptomic profiling was carried out of these cultures to measure oxygen-dependent gene expression using a reference-design microarray format.

INSTRUMENT(S): Infors-HT Labfors chemostat

ORGANISM(S): Escherichia coli

SUBMITTER: Matthew Rolfe

PROVIDER: E-MTAB-285 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations.

Rolfe Matthew D MD Ter Beek Alex A Graham Alison I AI Trotter Eleanor W EW Asif H M Shahzad HM Sanguinetti Guido G de Mattos Joost Teixeira JT Poole Robert K RK Green Jeffrey J

The Journal of biological chemistry 20110120 12

Oxygen availability is the major determinant of the metabolic modes adopted by Escherichia coli. Although much is known about E. coli gene expression and metabolism under fully aerobic and anaerobic conditions, the intermediate oxygen tensions that are encountered in natural niches are understudied. Here, for the first time, the transcript profiles of E. coli K-12 across the physiologically significant range of oxygen availabilities are described. These suggested a progressive switch to aerobic ...[more]

PMID: 21252224

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