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Unknown,Transcriptomics,Genomics,Proteomics

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RNA sequencing of mouse embryonic development

ABSTRACT: Transcriptional profiling of mouse embryos spanning the 8-cell morula stage to E5.5 postimplantation epiblast was performed using lineage-specific RNA-seq.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-2958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis.

Boroviak Thorsten T Loos Remco R Loos Remco R Lombard Patrick P Okahara Junko J Behr Rüdiger R Sasaki Erika E Nichols Jennifer J Smith Austin A Bertone Paul P

Developmental cell 20151101 3

Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment an ...[more]

PMID: 26555056

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