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Unknown,Transcriptomics,Genomics,Proteomics

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Histone ChIP NS5 Ascl1-ERT2

ABSTRACT: Here we performed a ChIP-seq experiment on a sample of adherent cultures of mouse neural stem cells (NS5 cell line) expressing an inducible version of the proneural factor Mash1/Ascl1 (Ascl1-ERT2) under normal growth conditions and after 24 hours of activation by 4-Hydroxytamoxifen. This resulted in the generation of a genome-wide map of histone modifications H3K27ac and H3K4me1.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Diogo Castro

PROVIDER: E-MTAB-3104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.

Raposo Alexandre A S F AASF Vasconcelos Francisca F FF Drechsel Daniela D Marie Corentine C Johnston Caroline C Dolle Dirk D Bithell Angela A Gillotin Sébastien S van den Berg Debbie L C DLC Ettwiller Laurence L Flicek Paul P Crawford Gregory E GE Parras Carlos M CM Berninger Benedikt B Buckley Noel J NJ Guillemot François F Castro Diogo S DS

Cell reports 20150305 9

The proneural transcription factor Ascl1 coordinates gene expression in both proliferating and differentiating progenitors along the neuronal lineage. Here, we used a cellular model of neurogenesis to investigate how Ascl1 interacts with the chromatin landscape to regulate gene expression when promoting neuronal differentiation. We find that Ascl1 binding occurs mostly at distal enhancers and is associated with activation of gene transcription. Surprisingly, the accessibility of Ascl1 to its bin ...[more]

PMID: 25753420

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