MicroRNA 140-5p is associated with the pathogenesis of multiple sclerosis

ABSTRACT: In order to assess potential alterations in microRNA expression in MS patients, we first applied microRNA array to screen the expression profile of 7 controls and 6 patients in the peripheral blood mononuclear cells (PBMC) PBMC when compared to age- and sex-matched controls Peripheral blood mononuclear cells (PBMC) were prepared by density gradient centrifugation using Nycoprep 1.077 medium (Accurate Chemical & Scientific Corp, Westbury, NY, USA). Total RNA including microRNAs were extracted using miRNeasy mini kit (Qiagen, Germantown, MD, USA) and subjected to microRNA high-throughput array with Affymetrix platform. MicroRNA array in Affymetrix 3.0 platform. Quantitative real-time PCR (QPCR) was applied for the validation and amplification of the test samples. Relative fold expression values were calculated based on the expression of Snord96A gene as an endogenous control. Microarray data are available in the ArrayExpress database (HUwww.ebi.ac.uk/arrayexpressUH) under accession number E-MEXP-4673.

ORGANISM(S): Homo sapiens

SUBMITTER: Elizabeth Zumbrun 

PROVIDER: E-MTAB-3437 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress