Exploiting native forces to capture chromosome conformation in mammalian cell nuclei
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ABSTRACT: Here, we developed a novel chromosome conformation capture (3C) method for capturing the 3D spatial contacts of endogenous genomic loci without a need for crosslinking. This method, i3C, was applied to multiple loci in two different human primary (ENCODE) cell lines, HUVEC and IMR90, and in the absence or presence of a proinflammatory stimulus (TNFalpha). Coupled to high throughput sequencing on an Illumina HiSeq200 platform, i3C generated aprrox. 8 million single-end reads per experiment.
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Homo sapiens
SUBMITTER: Argyris Papantonis
PROVIDER: E-MTAB-4719 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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