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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional regulation of human pterygial-derived fibroblasts in response to FCS and TGFbeta

ABSTRACT: Pterygium is an ocular disease that involves a fleshy growth from the conjunctiva across the cornea. It demonstrates accelerated fibroblastic proliferation, matrix deposition and neovascularisation, which is closely linked to inflammation. Growth factor levels are elevated in human pterygium through the enriched blood supply and these are believed to drive physiological events. For this reason, human fibroblasts derived from surgically removed pterygium samples (obtained with full ethical approval) were treated for 24 hours with 10% foetal calf serum (FCS), to mimic an enriched growth factor environment, Transforming growth factor beta2 (TGFÎ²2), which is often associated with fibrosis and has previously been detected in pterygium, or maintained in serum-free control medium (Eagle's minimum essential medium (EMEM)). To ascertain gross changes in gene expression as a consequence of these treatment groups a HumanHT-12 v4 Expression BeadChip Kit was employed.

ORGANISM(S): Homo sapiens

SUBMITTER: Ian Michael Wormstone

PROVIDER: E-MTAB-4871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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