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Unknown,Transcriptomics,Genomics,Proteomics

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Oryza sativa Japonica strain:EFR-XA21 | cultivar:Kitaake Transcriptome or Gene expression

ABSTRACT: We analyzed the transcriptomic profile of EFR:XA21:GFP rice lines treated with elf18 to identify genes differentially regulated during this response. We sequenced cDNA from EFR:XA21:GFP leaves treated with 500 nM elf18 for 0.5, 1, 3, 6, and 12 h. We also included untreated EFR:XA21:GFP and Kitaake as controls. Note: All samples in SRA were assigned the same sample accession (SRS843490). This is incorrect as there are different samples, hence âSource Nameâ was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Pamela Ronald

PROVIDER: E-MTAB-5170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.

Thomas Nicholas C NC Schwessinger Benjamin B Liu Furong F Chen Huamin H Wei Tong T Nguyen Yen P YP Shaker Isaac W F IWF Ronald Pamela C PC

PeerJ 20160928

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both ...[more]

PMID: 27703843

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