ABSTRACT: mRNA from tumor cells, tumor associated macrophages and tumor associated T cells from high grade serous ovarian carcinoma patients was sequenced.
Project description:Transcriptome of high grade serous ovarian carcinoma isolated from patient acites and associated macrophages was analyzed using RNASeq.
Project description:Ascites-associated macrophages were collected from human ovarian carcinoma patients and their expression profiles determined via Agilent microarrays. In addition, monocyte derived macrophages from healthy donors were differentiated ex-vivo with MCSF and GMCSF and their expression profiles determined as well.
Project description:The transcriptome of serous ovarian cancer tumor associated macrophages was characterized. Additionally, their transcription response to PPARD agonists and inverse agonists in cell culture was analyzed.
Project description:Primary cells from high grade serous ovarian carcinoma patients, derived from ascites and omentum were sequenced to study signaling networks.
Project description:Chemotherapies have been shown to enhance anti-tumor immunity, but whether chemotherapies affect tumor-associated macrophages (TAMs) is still unclear. We found TAMs is different before and after chemotherapy by comparing proteomic profiles of TAMs before and after chemotherapy.
Project description:The secretomes of tumor cells, and macrophages from high grade serous ovarian carcinoma from the ascites of patients undergoing primary surgery were analysed after 24h culture (ex vivo) via obitrap.
Project description:CAFs, ascTAMs and M1- and TAM-like-MDMs were treated with 1 μM of the Prostacyclin analoga MRE and DMSO (as solvent control). The aim of this experiment is to see which effect MRE (Prostacyclinanaloga) has on RNA expression (activation of signaling pathways). Background: Prostacyclin receptor expression is elevated in TAMs. Prostacyclinsynthase expression was detected mainly in CAFs. (