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Chromatin immunoprecipitation of Twist protein during embryonic development in Drosophila

ABSTRACT: Genomewide mapping of Drosophila Twist protein binding during embryonic development. Two consecutive timepoints (2-4 and 4-6 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Twist protein. Additionally, preimmune-serum was used as a control. The enriched DNA was hybridized to custom designed tiling arrays optimized for assaying all E-box motifs outside repetitive or coding regions of the Drosophila melanogaster genome (average gap size = 290 bps).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot 

PROVIDER: E-TABM-159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A core transcriptional network for early mesoderm development in Drosophila melanogaster.

Sandmann Thomas T   Girardot Charles C   Brehme Marc M   Tongprasit Waraporn W   Stolc Viktor V   Furlong Eileen E M EE  

Genes & development 20070201 4

Embryogenesis is controlled by large gene-regulatory networks, which generate spatially and temporally refined patterns of gene expression. Here, we report the characteristics of the regulatory network orchestrating early mesodermal development in the fruitfly Drosophila, where the transcription factor Twist is both necessary and sufficient to drive development. Through the integration of chromatin immunoprecipitation followed by microarray analysis (ChIP-on-chip) experiments during discrete tim  ...[more]

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