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Chromatin immunoprecipitation of Drosophila to identify muscle differentiation factor Mef2 protein binding during embryonic development

ABSTRACT: Genomewide mapping of D. melanogaster the muscle differentiation factor Mef2 protein binding during embryonic development. Five consecutive timepoints (2-4, 4-6, 6-8, 8-10, 10-12 hrs after egg-laying) were assayed in two independent repeats each. Two different antibodies were used to precipitate the Mef2 protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-TABM-649 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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