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Identification by ChIP-Seq of the binding sites of the extracytoplasmic function (ECF) sigma factor SigE in the genome of Streptomyces coelicolor A3(2) M600.

ABSTRACT: SigE is a sigma factor found in Streptomyces species and is the key regulator of cell envelope stress response in Streptomyces coelicolor. This ChIP-Seq experiment was carried out to determine the binding sites of SigE under conditions of cell envelope stress induced by 10 ug / ml vancomycin for 30 minutes. A 3xFLAG tagged SigE was introduced in a SigE deletion strain and ChIP-Seq was carried out using M2 (Sigma Aldrich A2220) gel suspension. Non-immunoprecipitated (total) DNA preparations were used for background determination and the wild type M600 was used as the negative control.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Govind Chandra

PROVIDER: E-MTAB-7827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Defining the regulon of genes controlled by σE , a key regulator of the cell envelope stress response in Streptomyces coelicolor.

Tran Ngat T NT Huang Xiaoluo X Hong Hee-Jeon HJ Bush Matthew J MJ Chandra Govind G Pinto Daniela D Bibb Maureen J MJ Hutchings Matthew I MI Mascher Thorsten T Buttner Mark J MJ

Molecular microbiology 20190429 2

The extracytoplasmic function (ECF) σ factor, σE , is a key regulator of the cell envelope stress response in Streptomyces coelicolor. Although its role in maintaining cell wall integrity has been known for over a decade, a comprehensive analysis of the genes under its control has not been undertaken. Here, using a combination of chromatin immunoprecipitation-sequencing (ChIP-seq), microarray transcriptional profiling and bioinformatic analysis, we attempt to define the σE ...[more]

PMID: 30907454

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