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Timecourse analysis of fission yeast CSL mutants treated with hydrogen peroxide

ABSTRACT: Fission yeast CSL mutants (cbf11 and cbf12 knock-outs) show altered sensitivity to hydrogen peroxide. To help elucidate the molecular basis of this phenomenon, we analyzed wild-type and mutant transcriptomes before stress and as a timecourse after treatment with hydrogen peroxide. Cells were grown to exponential phase in YES medium, untreated samples were collected (time 0), cells were treated with 0.74 mM hydrogen peroxide and harvested after 15 and 60 min post treatment. Total RNA was isolated, labelled and hybridized to custom-made two-colour microarrays. Each sample was hybridized against reference created by pooling equal aliquots from all 9 samples.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Martin Převorovský

PROVIDER: E-MTAB-6761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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