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RNA-seq of human near-haploid cell line HAP1 with CRISPR-edited frameshift mutations

ABSTRACT: The aim of the experiment is to assess the robustness of CRISPR-Cas9 based genetic engineering. Using RNA sequencing, we are able to quantify the abundance of transcripts corresponding to CRISPR-targets and thus the degree of nonsense mediated decay.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Frederik Ziebell

PROVIDER: E-MTAB-7061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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