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Single cell RNA sequencing of putative AIRE+ dendritic cells from human paediatric tonsillitis

ABSTRACT: Characterisation and identification of putative human extra-thymic AIRE-expressing cells from paediatric tonsillitis samples using single -cell transcriptome profiling.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Mike Morgan

PROVIDER: E-MTAB-7381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Maturing Human CD127+ CCR7+ PDL1+ Dendritic Cells Express AIRE in the Absence of Tissue Restricted Antigens.

Fergusson Joannah R JR Morgan Michael D MD Bruchard Melanie M Huitema Leonie L Heesters Balthasar A BA van Unen Vincent V van Hamburg Jan Piet JP van der Wel Nicole N NN Picavet Daisy D Koning Frits F Tas Sander W SW Anderson Mark S MS Marioni John C JC Holländer Georg A GA Spits Hergen H

Frontiers in immunology 20190114

Expression of the Autoimmune regulator (AIRE) outside of the thymus has long been suggested in both humans and mice, but the cellular source in humans has remained undefined. Here we identify AIRE expression in human tonsils and extensively analyzed these "extra-thymic AIRE expressing cells" (eTACs) using combinations of flow cytometry, CyTOF and single cell RNA-sequencing. We identified AIRE+ cells as dendritic cells (DCs) with a mature and migratory phenotype including high levels of antigen p ...[more]

PMID: 30692988

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