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PacBio Iso-Seq transcriptome sequencing data of multiplexed maize cDNA

ABSTRACT: Full-Length cDNA transcriptome (Iso-Seq) data sequenced on the PacBio Sequel system using 2.1 chemistry. Multiplexed cDNA library of 12 samples (3 tissues x 4 strains). Tissues: root, embryo, endosperm. Strains: B73, Ki11, B73xKi11, Ki11xB73.

INSTRUMENT(S): Sequel

ORGANISM(S): Zea mays

SUBMITTER: Bo Wang

PROVIDER: E-MTAB-7837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Variant phasing and haplotypic expression from long-read sequencing in maize.

Wang Bo B Tseng Elizabeth E Baybayan Primo P Eng Kevin K Regulski Michael M Jiao Yinping Y Wang Liya L Olson Andrew A Chougule Kapeel K Buren Peter Van PV Ware Doreen D

Communications biology 20200218 1

Haplotype phasing maize genetic variants is important for genome interpretation, population genetic analysis and functional analysis of allelic activity. We performed an isoform-level phasing study using two maize inbred lines and their reciprocal crosses, based on single-molecule, full-length cDNA sequencing. To phase and analyze transcripts between hybrids and parents, we developed IsoPhase. Using this tool, we validated the majority of SNPs called against matching short-read data from embryo, ...[more]

PMID: 32071408

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