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Single cell RNA-seq of in vitro cultured human embryos

ABSTRACT: Human embryogenesis is characterised by a series of coordinated cell fate specification events. Upon implantation on day 7, the three cell lineages forming the human blastocyst undergo major morphogenetic and transcriptomic changes. Using single-cell RNA sequencing, we reconstruct the molecular events driving post-implantation development of the human embryo at day 9 and 11. Our study provides the first molecular map of gene expression patterns in early post-implantation human embryos, unveiling how signalling interactions between embryonic and extra-embryonic tissues drive human embryogenesis.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Tim Coorens

PROVIDER: E-MTAB-8060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre.

Molè Matteo A MA Coorens Tim H H THH Shahbazi Marta N MN Weberling Antonia A Weatherbee Bailey A T BAT Gantner Carlos W CW Sancho-Serra Carmen C Richardson Lucy L Drinkwater Abbie A Syed Najma N Engley Stephanie S Snell Philip P Christie Leila L Elder Kay K Campbell Alison A Fishel Simon S Behjati Sam S Vento-Tormo Roser R Zernicka-Goetz Magdalena M

Nature communications 20210617 1

Following implantation, the human embryo undergoes major morphogenetic transformations that establish the future body plan. While the molecular events underpinning this process are established in mice, they remain unknown in humans. Here we characterise key events of human embryo morphogenesis, in the period between implantation and gastrulation, using single-cell analyses and functional studies. First, the embryonic epiblast cells transition through different pluripotent states and act as a sou ...[more]

PMID: 34140473

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