RNA-Seq of Drosophila melanogaster to count absolute number of molecules using unique molecular identifiers
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Teemu Kivioja
PROVIDER: E-MTAB-816 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Counting absolute numbers of molecules using unique molecular identifiers.
Kivioja Teemu T Vähärautio Anna A Karlsson Kasper K Bonke Martin M Enge Martin M Linnarsson Sten S Taipale Jussi J
Nature methods 20111120 1
Counting individual RNA or DNA molecules is difficult because they are hard to copy quantitatively for detection. To overcome this limitation, we applied unique molecular identifiers (UMIs), which make each molecule in a population distinct, to genome-scale human karyotyping and mRNA sequencing in Drosophila melanogaster. Use of this method can improve accuracy of almost any next-generation sequencing method, including chromatin immunoprecipitation-sequencing, genome assembly, diagnostics and ma ...[more]
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