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Unknown,Transcriptomics,Genomics,Proteomics

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RNAseq of young adult C. elegans animals fed on three different bacterial diets, E. coli OP50, B. subtilis PXN21 and a mixture of both

ABSTRACT: To uncover the host response pathways that are modified by a B. subtilis diet and that may provide the protective effect against α-synuclein aggregation, we performed comparative global transcriptomics analysis using next generation RNA sequencing (RNA-seq). We compared young adult animals fed on three different diets, E. coli OP50 and B. subtilis PXN21 lawns, consisting of both spores and vegetative cells, and a mixture of both bacteria.

INSTRUMENT(S): Illumina HiSeq 4000, -

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Andres Romanowski

PROVIDER: E-MTAB-8164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Probiotic Bacillus subtilis Protects against α-Synuclein Aggregation in C. elegans.

Goya María Eugenia ME Xue Feng F Sampedro-Torres-Quevedo Cristina C Arnaouteli Sofia S Riquelme-Dominguez Lourdes L Romanowski Andrés A Brydon Jack J Ball Kathryn L KL Stanley-Wall Nicola R NR Doitsidou Maria M

Cell reports 20200101 2

Recent discoveries have implicated the gut microbiome in the progression and severity of Parkinson's disease; however, how gut bacteria affect such neurodegenerative disorders remains unclear. Here, we report that the Bacillus subtilis probiotic strain PXN21 inhibits α-synuclein aggregation and clears preformed aggregates in an established Caenorhabditis elegans model of synucleinopathy. This protection is seen in young and aging animals and is partly mediated by DAF-16. Multiple B. subtilis str ...[more]

PMID: 31940482

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