RNA-seq of wild-type Salmonella Typhimurium SL1344 in the presence and absence of chlorpromazine or Phe-Arg-Beta-Napthylamide
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ABSTRACT: The aim of this experiment was to determine the changes in transcription caused by inhibition of efflux pumps by the Phe-Arg-Beta-Naptylamide (PABN) and chlorpromazine. Four biological replicates per condition were grown to mid-log phase in MOPS minimal media (OD600) and were exposed to the known efflux inhibitor PABN, and the proposed efflux inhibitor chlorpromazine at 100 µg/ml and 50 µg/ml, respectively. Exposure lasted for two hours. Total RNA was extracted and DNase-treated. The samples were sent to BGI, Hong Kong for sequencing by Illumina Hiseq 4000.
INSTRUMENT(S): Illumina HiSeq 4000
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium
SUBMITTER: Laura Piddock
PROVIDER: E-MTAB-8190 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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