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RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

ABSTRACT: Induced pluripotent stem cells were differentiated to brain microvascular endothelial cells (BMECs) using previously published protocols with minor changes. RNA was collected from purified BMECs after barrier induction and submitted for sequencing.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Lawrence Hsu

PROVIDER: E-MTAB-8802 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs.

Neal Emma H EH Marinelli Nicholas A NA Shi Yajuan Y McClatchey P Mason PM Balotin Kylie M KM Gullett Dalton R DR Hagerla Kameron A KA Bowman Aaron B AB Ess Kevin C KC Wikswo John P JP Lippmann Ethan S ES

Stem cell reports 20190601 6

Human induced pluripotent stem cell (iPSC)-derived developmental lineages are key tools for in vitro mechanistic interrogations, drug discovery, and disease modeling. iPSCs have previously been differentiated to endothelial cells with blood-brain barrier (BBB) properties, as defined by high transendothelial electrical resistance (TEER), low passive permeability, and active transporter functions. Typical protocols use undefined components, which impart unacceptable variability on the differentiat ...[more]

PMID: 31189096

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