Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

35 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Induction of AopACT in adult Drosophila abdominal fat body

ABSTRACT: Transcript profiling of adult abdominal fat body in S1106>AopACT females after 5 days of induction of transgene expression by RU486 feeding.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nazif Alic

PROVIDER: E-MTAB-927 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Interplay of dFOXO and two ETS-family transcription factors determines lifespan in Drosophila melanogaster.

Alic Nazif N Giannakou Maria E ME Papatheodorou Irene I Hoddinott Matthew P MP Andrews T Daniel TD Bolukbasi Ekin E Partridge Linda L

PLoS genetics 20140918 9

Forkhead box O (FoxO) transcription factors (TFs) are key drivers of complex transcriptional programmes that determine animal lifespan. FoxOs regulate a number of other TFs, but how these TFs in turn might mediate the anti-ageing programmes orchestrated by FoxOs in vivo is unclear. Here, we identify an E-twenty six (ETS)-family transcriptional repressor, Anterior open (Aop), as regulated by the single Drosophila melanogaster FoxO (dFOXO) in the adult gut. AOP, the functional orthologue of the hu ...[more]

PMID: 25232726

Similar Datasets

Transcription profiling by array of adult Drosophila melanogaster gut and fat body in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

Project description:Transcription profiling by array of adult abdominal/thorasic fat body or midgut in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

2014-08-18 | E-MTAB-1020 | biostudies-arrayexpress

Feedback from dFOXO in the gut and fat body

Project description:Whole fly transcript profiles of changes upon induction of dFOXO in the gut and fat body using S1106>dFOXO flies and how this response changes when the feedback to foxo is removed in the foxo null background

2014-02-05 | E-MTAB-1232 | biostudies-arrayexpress

ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

Project description:ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

2014-08-18 | E-MTAB-1021 | biostudies-arrayexpress

AopACT binding in adult gut and fat body upon its induction in Drosophila melanogaster

Project description:ChIP-chip on FLAG-tagged AopACT expressed in adult gut and fat body.

2014-08-18 | E-MTAB-1306 | biostudies-arrayexpress

Genome-Wide Progesterone Receptor Binding: Cell Type-Specific and Shared Mechanisms in Uterine Fibroids and Breast Cancer

Project description:Analysis of genes regulated by RU486 (an progesterone antagonist) in human breast cancer T47D cells and human uterine leiomyoma smooth muscle cells. The hypothesis is that RU486 inhibits tumor growth by inactivating the transcription of multiple genes which trigger critical signaling pathways to induce tumorigenesis in both breast caner and uterine leomyoma. Tissue-specific and common patterns of gene regulation may determine the therapeutic effects of antiprogestins in uterine leiomyoma and breast cancer. Total RNA isolated from T47D cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells. Total RNA isolated from uterine leiomyoma cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells.

2011-07-22 | E-GEOD-30871 | biostudies-arrayexpress

Genome-Wide Progesterone Receptor Binding: Cell Type-Specific and Shared Mechanisms in Uterine Fibroids and Breast Cancer (Expression BeadChip)

Project description:Analysis of genes regulated by RU486 (an progesterone antagonist) in human breast cancer T47D cells and human uterine leiomyoma smooth muscle cells. The hypothesis is that RU486 inhibits tumor growth by inactivating the transcription of multiple genes which trigger critical signaling pathways to induce tumorigenesis in both breast caner and uterine leomyoma. Tissue-specific and common patterns of gene regulation may determine the therapeutic effects of antiprogestins in uterine leiomyoma and breast cancer. Keywords: Expression profiling by array Total RNA isolated from T47D cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells. Total RNA isolated from uterine leiomyoma cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells.

2012-09-11 | E-GEOD-40725 | biostudies-arrayexpress

Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster

Project description:Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster. The experiment was performed to examine which parts of the transcriptional response to a reduction in insulin signalling in an adult female fly depend on the presence of the dfoxo transcription factor. Whole fly transcriptome was determined with flies over-expressing a dominant negative form of the insulin receptor, or the wild-type fly, in presence or absence of dfoxo.

2011-06-18 | E-TABM-757 | biostudies-arrayexpress

Genome-Wide Progesterone Receptor Binding: Cell Type-Specific and Shared Mechanisms in Uterine Fibroids and Breast Cancer (ChIP-Seq)

Project description:Analysis of genes regulated by RU486 (an progesterone antagonist) in human breast cancer T47D cells and human uterine leiomyoma smooth muscle cells. The hypothesis is that RU486 inhibits tumor growth by inactivating the transcription of multiple genes which trigger critical signaling pathways to induce tumorigenesis in both breast caner and uterine leomyoma. Tissue-specific and common patterns of gene regulation may determine the therapeutic effects of antiprogestins in uterine leiomyoma and breast cancer. We applied ChIP-seq to identify PR-interaction sites in T47D breast cancer cells and primary uterine leiomyoma cells treated with RU486.

2012-09-11 | E-GEOD-40724 | biostudies-arrayexpress

Corticosterone regulation of mRNAs and and corresponding RU486 antagonism in astrocytes in vitro

Project description:Analysis of mRNA gene expression changes mediated by natural glucocorticoid ligand corticosterone in mouse cortical primary astrocyte cultures; also, parallel analysis of RU486 (mifepristone) antagonism of corticosterone-mediated mRNA changes. Total RNA obtained from astrocyte cultures subjected to 2, 4, or 6 hours of (1) 100nM corticosterone alone, (2) 500nM RU486 alone, or (3) 100nM corticosterone + 500nM RU486 exposure compared to vehicle control astrocyte cultures.

2013-01-09 | E-GEOD-39270 | biostudies-arrayexpress

Transcriptional profiling of fruit flies overexpressing the Atg1 gene

Project description:Overexpression of Atg1 using either weaker CGGAL4 or stronger HRGAL4 driver. Both drivers have same expression pattern and are specific for intestine, Malpighian tubules and fat body of the fruit fly Drosophila melanogaster. Weaker Atg1 overexpression results in longer lifespan whereas stronger Atg1 overexpression shortens lifespan compared to controls. Overexpressor lines were combined with tubGAL80ts, which is a temperature sensitive GAL4 repressor. This enabled induction of Atg1 overexpression in day-2 adult fly, thereby bypassing development and any potential developmental effects that Atg1 might have. Fly eggs of appropriate genotype were raised under standard density at 18C and then emerged flies were switched to 27C at day 2 of adulthood for Atg1 overexpression, and were kept at 27C onwards for longevity analyses. Dissected tissue was collected for the transcriptomic analysis at two weeks of age.

2020-07-15 | E-MTAB-9391 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data