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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type and ZakA and GskA knock-out Dictyostelium discoideum to investigate the Zak1-GSK-3 signalling pathway

ABSTRACT: GskA, the Dictyostelium GSK-3 orthologue, is modified and activated by the dual-specificity tyrosine kinase Zak1 and the two kinases form part of a signalling pathway that responds to extracellular cAMP. We identify potential cellular effectors for the two kinases by analysing their null mutants. There are proteins and mRNAs that are altered in abundance in only one or other of the two mutants, indicating that each kinase has some unique functions. However, proteomic and micro-array analysis respectively identified 3 proteins and 37 genes that are similarly mis-regulated in both mutant strains. The positive correlation between the array data and the proteomics data is consistent with the Zak1-GskA signalling pathway functioning by directly or indirectly regulating gene expression. The discoidin 1 genes are positively regulated by the pathway while the abundance of the H5 protein is negatively regulated. Two of the targets, H5 and discoidin 1, are well-characterised markers for early development, indicating that the Zak1-GskA pathway plays a role in development earlier than previously observed.

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Matloob Qureshi

PROVIDER: E-SGRP-4 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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