ChIP-chip by array of Arabidopsis endosperm nuclei expressing a PHE1::PHE1-GFP reporter using H3K27me3 and IgG antibodies
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Lars Hennig
PROVIDER: E-TABM-1008 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:The conserved Notch pathway functions in diverse developmental and disease-related processes, requiring mechanisms to ensure appropriate target-selection and gene activation in each context. To investigate, we partitioned Drosophila chromatin into different states, based on histone modifications, establishing the preferred chromatin conditions for binding of CSL, the Notch pathway transcription factor. While most histone modifications were unchanged by CSL binding or Notch activation, rapid changes in H3K56 acetylation occurred at Notch regulated-enhancers. This modification extended over large regions, required the histone acetyl-transferase CBP and was independent of transcription. Such rapid changes in H3K56 acetylation are a conserved indicator of enhancer activation, also occurring at mammalian Notch-regulated Hey1 and at Drosophila ecdysone-regulated genes. This core histone modification may therefore underpin the changes in chromatin accessibility needed to promote transcription following signaling activation. Su(H) profile of BG3 cells in control condition and EGTA treated condition. In total 8 samples, 4 replicates of Su(H) ChIP in hbss condition and 4 replicates of Su(H) ChIP in EGTA treated BG3 cells.
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