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A Dictyostelium SH2 domain adaptor protein required for correct DIF-1 signaling and pattern formation


ABSTRACT: When developing in the light slug formation in LrrB null (lrrB-) mutants is delayed, relative to the parental strain, and the slugs are highly defective in phototaxis and thermotaxis. In the dark the mutant arrests development as an elongated mound, in a novel process we term dark stalling. The developmental and phototaxis defects are cell autonomous and marker analysis shows that the pstO prestalk sub-region of the slug is aberrant in the lrrB- mutant. Expression profiling reveals many other alterations in gene expression in lrrB- slugs compared to the wild-type parental Ax2 line, including greatly reduced expression of the ecmB gene.

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Gareth Bloomfield 

PROVIDER: E-TABM-1090 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Dictyostelium SH2 adaptor protein required for correct DIF-1 signaling and pattern formation.

Sugden Christopher C   Ross Susan S   Annesley Sarah J SJ   Cole Christian C   Bloomfield Gareth G   Ivens Alasdair A   Skelton Jason J   Fisher Paul R PR   Barton Geoffrey G   Williams Jeffrey G JG  

Developmental biology 20110321 2


Dictyostelium is the only non-metazoan with functionally analyzed SH2 domains and studying them can give insights into their evolution and wider potential. LrrB has a novel domain configuration with leucine-rich repeat, 14-3-3 and SH2 protein-protein interaction modules. It is required for the correct expression of several specific genes in early development and here we characterize its role in later, multicellular development. During development in the light, slug formation in LrrB null (lrrB-)  ...[more]

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