Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparative genomic hybridization of DNA from humans from different populations in self-self hybridizations, replicate experiments and independent validations of copy number variations to assess array performance

ABSTRACT: Data generated in the validation of a large-insert clone DNA microarray covering the entire human genome in tiling path resolution, which we have used to identify copy number variation in human populations. Array performance was extensively tested by a series of validation assays. These included determining the hybridization characteristics of each individual clone on the array by chromosome specific add-in experiments. Estimation of data reproducibility and falsepositive/negative rates was carried out using self-self hybridizations, replicate experiments and independent validations of CNVs.

ORGANISM(S): Homo sapiens

SUBMITTER: Armand Valsesia

PROVIDER: E-TABM-123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Accurate and reliable high-throughput detection of copy number variation in the human genome.

Fiegler Heike H Redon Richard R Andrews Dan D Scott Carol C Andrews Robert R Carder Carol C Clark Richard R Dovey Oliver O Ellis Peter P Feuk Lars L French Lisa L Hunt Paul P Kalaitzopoulos Dimitrios D Larkin James J Montgomery Lyndal L Perry George H GH Plumb Bob W BW Porter Keith K Rigby Rachel E RE Rigler Diane D Valsesia Armand A Langford Cordelia C Humphray Sean J SJ Scherer Stephen W SW Lee Charles C Hurles Matthew E ME Carter Nigel P NP

Genome research 20061122 12

This study describes a new tool for accurate and reliable high-throughput detection of copy number variation in the human genome. We have constructed a large-insert clone DNA microarray covering the entire human genome in tiling path resolution that we have used to identify copy number variation in human populations. Crucial to this study has been the development of a robust array platform and analytic process for the automated identification of copy number variants (CNVs). The array consists of ...[more]

PMID: 17122085

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Dataset Information

Comparative genomic hybridization of DNA from humans from different populations in self-self hybridizations, replicate experiments and independent validations of copy number variations to assess array performance

Publications

Accurate and reliable high-throughput detection of copy number variation in the human genome.

Similar Datasets

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