Transcription profiling of eight different time periods of the Anopheles gambiae lifecycle

ABSTRACT: We conducted a genome wide survey of mosquito gene expression profiles during 8 different time periods of the Anopheles gambiae lifecycle. The timeperiods included embryos, E, collected 12-14 hours post oviposition (hpo), five larval samples, La-Le, with La collected approximately 48h hpo and the rest, Lb-Le, collected at 48 intervals thereafter, irrespective of larval size and instar, pupae, P, collected at the same time as Le samples (240 hpo) and freshly emerged adult females, F and males, M, collected 312 hpo. To investigate mosquito expression during development, we performed competitive two-dye hybridizations of experimental and standard reference RNA samples to MMC1 microarrays. MMC1 microarrays contain approximately 20,000 EST clones. Standard reference RNA was produced in vitro from the spotted ESTs and was utilized to provide consistent, non-zero reference values for almost all probes of the array. For the experiment, three biological replicates, corresponding to three successive mosquito generations and one technical (dye-swap) replicate was conducted.

ORGANISM(S): Anopheles gambiae

SUBMITTER: unknown MacCallum 

PROVIDER: E-TABM-186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress