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Transcription profiling of mouse hematopoetic progenitor cells with Flk12+ Ncad-, Flk12- Ncad+, Flk12+ Ncad+ or Flk12- Ncad- phenotypes

ABSTRACT: N-cadherin expression level distinguishes reserved versus primed states of hematopoietic stem cells

ORGANISM(S): Mus musculus

SUBMITTER: Ariel Paulson

PROVIDER: E-TABM-404 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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N-cadherin expression level distinguishes reserved versus primed states of hematopoietic stem cells.

Haug Jeffrey S JS He Xi C XC Grindley Justin C JC Wunderlich Joshua P JP Gaudenz Karin K Ross Jason T JT Paulson Ariel A Wagner Kathryn P KP Xie Yucai Y Zhu Ruihong R Yin Tong T Perry John M JM Hembree Mark J MJ Redenbaugh Erin P EP Radice Glenn L GL Seidel Christopher C Li Linheng L

Cell stem cell 20080401 4

Osteoblasts expressing the homophilic adhesion molecule N-cadherin form a hematopoietic stem cell (HSC) niche. Therefore, we examined how N-cadherin expression in HSCs relates to their function. We found that bone marrow (BM) cells highly expressing N-cadherin (N-cadherin(hi)) are not stem cells, being largely devoid of a Lineage(-)Sca1(+)cKit(+) population and unable to reconstitute hematopoietic lineages in irradiated recipient mice. Instead, long-term HSCs form distinct populations expressing ...[more]

PMID: 18397756

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