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Chromatin immunoprecipitation of Mef2 protein in Drosophila during embryonic development

ABSTRACT: Genomewide mapping of Drosophila Mef2 protein binding during embryonic development. Five consecutive timepoints (2-4, 4-6, 6-8, 8-10, 10-12 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Mef2 protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot 

PROVIDER: E-TABM-56 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A temporal map of transcription factor activity: mef2 directly regulates target genes at all stages of muscle development.

Sandmann Thomas T   Jensen Lars J LJ   Jakobsen Janus S JS   Karzynski Michal M MM   Eichenlaub Michael P MP   Bork Peer P   Furlong Eileen E M EE  

Developmental cell 20060601 6

Dissecting components of key transcriptional networks is essential for understanding complex developmental processes and phenotypes. Genetic studies have highlighted the role of members of the Mef2 family of transcription factors as essential regulators in myogenesis from flies to man. To understand how these transcription factors control diverse processes in muscle development, we have combined chromatin immunoprecipitation analysis with gene expression profiling to obtain a temporal map of Mef  ...[more]

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