Dataset Information

Nipah Virus Bangladesh Infection Elicits Organ-Specific Innate and Inflammatory Responses in the Marmoset Model.

ABSTRACT: The common marmoset (Callithrix jacchus) is increasingly recognized as an ideal nonhuman primate (NHP) at high biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all 4 infected marmosets. Three developed pulmonary edema and hemorrhage as well as multifocal hemorrhagic lymphadenopathy, while 1 recapitulated neurologic clinical manifestations and cardiomyopathy on gross pathology. Organ-specific innate and inflammatory responses were characterized by RNA sequencing in 6 different tissues from infected and control marmosets. Notably, a unique transcriptome was revealed in the brainstem of the marmoset exhibiting neurological signs. Our results provide a more comprehensive understanding of NiV pathogenesis in an accessible and novel NHP model, closely reflecting clinical disease as observed in NiV patients.

SUBMITTER: Stevens CS

PROVIDER: S-EPMC10469344 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Nipah Virus Bangladesh Infection Elicits Organ-Specific Innate and Inflammatory Responses in the Marmoset Model.

Stevens Christian S CS Lowry Jake J Juelich Terry T Atkins Colm C Johnson Kendra K Smith Jennifer K JK Panis Maryline M Ikegami Tetsuro T tenOever Benjamin B Freiberg Alexander N AN Lee Benhur B

The Journal of infectious diseases 20230801 5

The common marmoset (Callithrix jacchus) is increasingly recognized as an ideal nonhuman primate (NHP) at high biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all 4 infected marmosets. Three developed pulmonary edema and hemorrhage as well as multifocal he ...[more]

PMID: 36869692

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Project description:BackgroundCellulitis caused by Escherichia coli is a common cause of condemnation of broiler chickens at slaughter worldwide and is associated with economic losses and a possible negative impact on animal welfare. The study objective was to monitor clinical signs and immune responses after subcutaneous E. coli inoculation (1.1-1.8 × 107 CFU), aiming to induce cellulitis. Three groups of broiler chickens (n = 15/group) were inoculated with well-characterized E. coli strains (group A: ECA18 O24:H4/ST117 and group B: ECB11 O153:H9/ST38) or with saline (control) at 22 days-of-age. Clinical signs of disease, body weight and immune parameters were monitored until euthanasia 12-14 days after inoculation followed by post-mortem examination.ResultsThe daily weight gain of the inoculated chickens was significantly lower one day after inoculation compared to the controls. Seven (23%) of the inoculated chickens displayed clinical signs: ruffled feathers, mild weakness, open-beak breathing and/or reluctance to stand, of which two birds were euthanized and one bird died. Five chickens in group B were observed with bacteraemia, which lasted up to three days after inoculation for two chickens. A transient increase in chicken mannose receptor MRC1L-B expression on circulating monocytes was observed one day after inoculation in both E. coli inoculated groups, with a more pronounced increase in group B. On day 7 after inoculation, the in vitro adherence of heterophils, monocytes and thrombocytes to the inoculated strain was increased in group B. Antibody titers to the inoculation strains were increased in some chickens in both groups on days 7 and 14 after inoculation, with the highest titers in group B. Seven (47%) and 13 (87%) of the chickens in group A and B, respectively, were diagnosed with cellulitis at post-mortem examination. In most birds, lesions consisted of plaque-like material embedded in the subcutaneous tissue of the abdominal wall.ConclusionsInoculation of E. coli caused cellulitis and prompted a rapid activation/redistribution of circulating monocytes followed by antibody production. The responses were most pronounced in chickens inoculated with E. coli strain ECB11, presumably because of a higher virulence.

Dataset Information

Nipah Virus Bangladesh Infection Elicits Organ-Specific Innate and Inflammatory Responses in the Marmoset Model.

Publications

Nipah Virus Bangladesh Infection Elicits Organ-Specific Innate and Inflammatory Responses in the Marmoset Model.

Similar Datasets

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