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Fruit size control by a zinc finger protein regulating pericarp cell size in tomato.

ABSTRACT: Fruit size is largely defined by the number and size of cells in the fruit. Endoreduplication - a specialized cell cycle - is highly associated with cell expansion during tomato fruit growth. However, how endoreduplication coupled with cell size is regulated remains poorly understood. In this study, we identified a zinc finger gene SlPZF1 (Solanum lycopersicum PERICARP-ASSOCIATED ZINC FINGER PROTEIN 1) that was highly expressed in the pericarp of developing fruits. Plants with altered SlPZF1 expression produced smaller fruits due to the reduction in cell size associated with weakened endoreduplication. Overexpressing SlPZF1 delayed cell division phase by enhancing early expression of several key cell cycle regulators including SlCYCD3;1 and two plant specific mitotic cyclin-dependent protein kinase (SlCDKB1 and SlCDKB2) in the pericarp tissue. Furthermore, we identified 14 putative SlPZF1 interacting proteins (PZFIs) via yeast two hybrid screening. Several PZFIs, including Pre-mRNA-splicing factor (SlSMP1/PZFI4), PAPA-1-like conserved region family protein (PZFI6), Fanconi anemia complex components (PZFI3 and PZFI10) and bHLH transcription factor LONESOME HIGHWAY (SlLHW/PZFI14), are putatively involved in cell cycle regulation. Our results demonstrate that fruit growth in tomato requires balanced expression of the novel cell size regulator SlPZF1.

SUBMITTER: Zhao F

PROVIDER: S-EPMC10515234 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Fruit size control by a zinc finger protein regulating pericarp cell size in tomato.

Zhao Fangfang F Zhang Jiajing J Weng Lin L Li Meng M Wang Quanhua Q Xiao Han H

Molecular horticulture 20210814 1

Fruit size is largely defined by the number and size of cells in the fruit. Endoreduplication - a specialized cell cycle - is highly associated with cell expansion during tomato fruit growth. However, how endoreduplication coupled with cell size is regulated remains poorly understood. In this study, we identified a zinc finger gene SlPZF1 (Solanum lycopersicum PERICARP-ASSOCIATED ZINC FINGER PROTEIN 1) that was highly expressed in the pericarp of developing fruits. Plants with altered SlPZF1 exp ...[more]

PMID: 37789485

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Project description:Abscisic acid (ABA) regulates plant development and adaptation to environmental conditions. The ABA biosynthesis pathway in plants has been thoroughly elucidated; however, very few transcription factors directly regulating the expression of ABA biosynthetic genes have been identified. Here we show that the tomato (Solanum lycopersicum) zinc finger transcription factor SlZFP2, which is mainly expressed in developing fruits and axillary buds, negatively regulates ABA biosynthesis. Overexpression of SlZFP2 resulted in multiple phenotypic changes, including more branches, early flowering, delayed fruit ripening, lighter seeds and faster seed germination, whereas gene silencing by RNA interference (RNAi) caused poor fruit set and inhibited seed germination. Gene expression analysis showed that SlZFP2 represses ABA biosynthesis mainly through downregulation of the ABA biosynthetic genes SITIENS (SIT), FLACCA (FLC) and aldehyde oxidase SlAO1. SlZFP2 delays the onset of ripening through suppression of the ripening regulator COLORLESS NON-RIPENING (CNR). Using bacterial one hybrid screening and a selected amplification and binding assay we identified the (A/T)(G/C)TT repeat as the core binding sequence of SlZFP2. We further identified a large number of tomato genes containing putative SlZFP2 binding sites in their promoter regions. Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that SIT, FLC and SlAO1 are direct targets of SlZFP2 through binding to their promoter regions. We propose that SlZFP2 represents a novel negative regulator for fine tuning ABA biosynthesis during fruit development and provides a potentially valuable tool for dissecting the role of ABA in fruit ripening.To gain further insight on transcriptome changes regulated by SlZFP2, we sequenced a representative SlZFP2 RNAi line in LA1589 background and its nontransgenic sibling (WT) on a Miseq platform. The RNAi line 207 showed defected fruit set and ABA biosynthesis were chosen for profiling gene expression via RNA sequencing. Its nontransgenic sibling was served as controls. Three biological replicates were conducted.

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Fruit size control by a zinc finger protein regulating pericarp cell size in tomato.

Publications

Fruit size control by a zinc finger protein regulating pericarp cell size in tomato.

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