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Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp.

ABSTRACT: This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of amino acids during tomato fruit ripening (Snowden et al., 2015) and based on the procedure used by Betty and Smith (Bettey and Smith, 1993). Such vesicles may be useful in further studies into the dynamic transfer of metabolites across the tonoplast for storage and metabolism during tomato fruit development.

SUBMITTER: Snowden CJ

PROVIDER: S-EPMC5660614 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp.

Snowden Christopher J CJ Thomas Benjamin B Baxter Charles J CJ Smith J Andrew C JAC Sweetlove Lee J LJ

Bio-protocol 20151201 24

This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of amino acids during tomato fruit ripening (Snowden <i>et al</i>., 2015) and based on the procedure used by Betty and Smith (Bettey and Smith, 1993). Such vesicles may be useful in further studies into t ...[more]

PMID: 29085859

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