Ontology highlight
ABSTRACT:
SUBMITTER: Morimatsu K
PROVIDER: S-EPMC129330 | biostudies-literature | 2002 Sep
REPOSITORIES: biostudies-literature
Morimatsu Katsumi K Takahashi Masayuki M Nordén Bengt B
Proceedings of the National Academy of Sciences of the United States of America 20020822 18
Linear dichroism (LD) polarized-light spectroscopy is used to determine the arrangement of RecA in its large filamentous complex with DNA, active in homologous recombination. Angular orientation data for two tryptophan and seven tyrosine residues, deduced from differential LD of wild-type RecA vs. mutants that were engineered to attenuate the UV absorption of selected residues, revealed a rotation by some 40 degrees of the RecA subunits relative to the arrangement in crystal without DNA. In addi ...[more]