Ontology highlight
ABSTRACT:
SUBMITTER: Heck JA
PROVIDER: S-EPMC1413905 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
Heck Julie Akiko JA Argueso Juan Lucas JL Gemici Zekeriyya Z Reeves Richard Guy RG Bernard Ann A Aquadro Charles F CF Alani Eric E
Proceedings of the National Academy of Sciences of the United States of America 20060221 9
In budding yeast, the MLH1-PMS1 heterodimer is the major MutL homolog complex that acts to repair mismatches arising during DNA replication. Using a highly sensitive mutator assay, we observed that Saccharomyces cerevisiae strains bearing the S288c-strain-derived MLH1 gene and the SK1-strain-derived PMS1 gene displayed elevated mutation rates that conferred a long-term fitness cost. Dissection of this negative epistatic interaction using S288c-SK1 chimeras revealed that a single amino acid polym ...[more]