Ontology highlight
ABSTRACT:
SUBMITTER: Sundheim O
PROVIDER: S-EPMC1523172 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Sundheim Ottar O Vågbø Cathrine B CB Bjørås Magnar M Sousa Mirta M L MM Talstad Vivi V Aas Per A PA Drabløs Finn F Krokan Hans E HE Tainer John A JA Slupphaug Geir G
The EMBO journal 20060706 14
Methylating agents are ubiquitous in the environment, and central in cancer therapy. The 1-methyladenine and 3-methylcytosine lesions in DNA/RNA contribute to the cytotoxicity of such agents. These lesions are directly reversed by ABH3 (hABH3) in humans and AlkB in Escherichia coli. Here, we report the structure of the hABH3 catalytic core in complex with iron and 2-oxoglutarate (2OG) at 1.5 A resolution and analyse key site-directed mutants. The hABH3 structure reveals the beta-strand jelly-rol ...[more]