Unknown

Dataset Information

0

Generation of heavy-chain-only antibodies in mice.

ABSTRACT: We have generated transgenic mice containing hybrid llama/human antibody loci that contain two llama variable regions and the human D, J, and Cmu and/or Cgamma constant regions. Such loci rearrange productively and rescue B cell development efficiently without LC rearrangement. Heavy-chain-only antibodies (HCAb) are expressed at high levels, provided that the CH1 domain is deleted from the constant regions. HCAb production does not require an IgM stage for effective pre-B cell signaling. Antigen-specific heavy-chain-only IgM or IgGs are produced upon immunization. The IgG is dimeric, whereas IgM is multimeric. The chimeric HCAb loci are subject to allelic exclusion, but several copies of the transgenic locus can be rearranged and expressed successfully on the same allele in the same cell. Such cells are not subject to negative selection. The mice produce a full antibody repertoire and provide a previously undescribed avenue to produce specific human HCAb in the future.

SUBMITTER: Janssens R 

PROVIDER: S-EPMC1586177 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Generation of heavy-chain-only antibodies in mice.

Janssens Rick R   Dekker Sylvia S   Hendriks Rudi W RW   Panayotou George G   van Remoortere Alexandra A   San John Kong-a JK   Grosveld Frank F   Drabek Dubravka D  

Proceedings of the National Academy of Sciences of the United States of America 20061002 41

We have generated transgenic mice containing hybrid llama/human antibody loci that contain two llama variable regions and the human D, J, and Cmu and/or Cgamma constant regions. Such loci rearrange productively and rescue B cell development efficiently without LC rearrangement. Heavy-chain-only antibodies (HCAb) are expressed at high levels, provided that the CH1 domain is deleted from the constant regions. HCAb production does not require an IgM stage for effective pre-B cell signaling. Antigen  ...[more]

Similar Datasets

Unknown Heavy chain-only antibodies are spontaneously produced in light chain-deficient mice.
| S-EPMC2150980 | biostudies-literature
Unknown Antibodies in a heavy chain knock-in mouse exhibit characteristics of early heavy chain rearrangement.
| S-EPMC2700953 | biostudies-literature
Unknown Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies.
| S-EPMC240713 | biostudies-literature
Unknown Reprogramming human B cells with custom heavy chain antibodies.
| S-EPMC10327003 | biostudies-literature
Unknown Multispecific Antibody Development Platform Based on Human Heavy Chain Antibodies.
| S-EPMC6330309 | biostudies-literature
Unknown One-step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock-in mice.
| S-EPMC6138433 | biostudies-literature
Unknown Immunoglobulin heavy chain variable region genes contribute to the induction of thyroid-stimulating antibodies in recombinant inbred mice.
| S-EPMC4108286 | biostudies-literature
Unknown Production of Autoreactive Heavy Chain-Only Antibodies in Systemic Lupus Erythematosus.
| S-EPMC7214812 | biostudies-literature
Unknown Development of polyclonal heavy chain antibodies targeting programmed death ligand-1.
| S-EPMC10298837 | biostudies-literature
Unknown Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection.
| S-EPMC6082650 | biostudies-literature