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Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage.

ABSTRACT: REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of REV1 with ubiquitin requires the ubiquitin-binding motifs (UBMs) located at the C terminus of REV1. The UBMs also mediate the enhanced association between monoubiquitylated PCNA and REV1. In cells exposed to UV radiation, the association of REV1 with replication foci is dependent on functional UBMs. The UBMs of REV1 are shown to contribute to DNA damage tolerance and damage-induced mutagenesis in vivo.

SUBMITTER: Guo C

PROVIDER: S-EPMC1636806 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature

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Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage.

Guo Caixia C Tang Tie-Shan TS Bienko Marzena M Parker Joanne L JL Bielen Aleksandra B AB Sonoda Eiichiro E Takeda Shunichi S Ulrich Helle D HD Dikic Ivan I Friedberg Errol C EC

Molecular and cellular biology 20060918 23

REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of REV1 with ubiquitin requires the ubiquitin-binding motifs (UBMs) located at the C terminus of REV1. The UBMs also mediate the enhanc ...[more]

PMID: 16982685

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Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance.

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