Gene expression profiling in single cells within tissue.
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ABSTRACT:
SUBMITTER: Capodieci P
PROVIDER: S-EPMC1976602 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
Publications
Gene expression profiling in single cells within tissue.
Capodieci Paola P Donovan Michael M Buchinsky Heidi H Jeffers Yusuf Y Cordon-Cardo Carlos C Gerald William W Edelson Jon J Shenoy Shailesh M SM Singer Robert H RH
Nature methods 20050901 9
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures. ...[more]
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