Ontology highlight
ABSTRACT:
SUBMITTER: Kholod N
PROVIDER: S-EPMC2049055 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Kholod Natalia N Boniver Jacques J Delvenne Philippe P
The Journal of molecular diagnostics : JMD 20071004 5
The identification of gene promoter methylation is a useful tool for the molecular diagnosis of human diseases. We have developed a new PCR-based technique for detecting the methylation status of CpG islands of gene promoters. This new method, named methyl-sensitive dimethyl sulfoxide-PCR (Ms-DMSO-PCR), is based on the finding that methylated and unmethylated DNAs show a different sensitivity to the amount of DMSO used in the PCR reaction. For the amplification of methylated DNA, more DMSO is re ...[more]