Dataset Information

Seroepidemiology of group I human coronaviruses in children.

ABSTRACT: Background: Recently, several new human coronaviruses have been identified.

Objectives: To define the seroepidemiology of group I human coronaviruses.

Study design: A recombinant protein enzyme linked immunosorbent assay (ELISA) based on portions of the nucleocapsid protein of group I human coronaviruses was developed and was used to screen serum from 243 children and young adults.

Results: For HCoV-229E, the percentages of seropositive individuals were 57.1% for infants <2 months old; 38.9% for infants 2-3 months old; 4.7% for infants 4-5 months old; 42.9-50.0% for infants 6-12 months old; 34.8-62.5% for individuals 1-20 years old. For HCoV-NL63, the percentages of seropositive individuals were 45.2% for infants <2 months old; 11.1% for infants 2-3 months old; 4.7% for infants 4-5 months old; 28.6-40.0% for infants 6-12 months old; 25.0-70.3% for individuals 1-20 years old.

Conclusions: Infection with these viruses is common in childhood though the prevalence of these viruses may vary from year to year.

SUBMITTER: Shao X

PROVIDER: S-EPMC2100388 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Seroepidemiology of group I human coronaviruses in children.

Shao Xiuping X Guo Xiaojie X Esper Frank F Weibel Carla C Kahn Jeffrey S JS

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20070921 3

<h4>Background</h4>Recently, several new human coronaviruses have been identified.<h4>Objectives</h4>To define the seroepidemiology of group I human coronaviruses.<h4>Study design</h4>A recombinant protein enzyme linked immunosorbent assay (ELISA) based on portions of the nucleocapsid protein of group I human coronaviruses was developed and was used to screen serum from 243 children and young adults.<h4>Results</h4>For HCoV-229E, the percentages of seropositive individuals were 57.1% for infants ...[more]

PMID: 17889596

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Project description:BackgroundMore than a decade after the outbreak of human coronaviruses (HCoVs) SARS in Guangdong province and Hong Kong SAR of China in 2002, there is still no reoccurrence, but the evolution and recombination of the coronaviruses in this region are still unknown. Therefore, surveillance on the prevalence and the virus variation of HCoVs circulation in this region is conducted.MethodsA total of 3298 nasopharyngeal swabs samples were collected from cross-border children (<6 years, crossing border between Southern China and Hong Kong SAR) showing symptoms of respiratory tract infection, such as fever (body temperature > 37.5 °C), from 2014 May to 2015 Dec. Viral nucleic acids were analyzed and sequenced to study the prevalence and genetic diversity of the four human coronaviruses. The statistical significance of the data was evaluated with Fisher chi-square test.Results78 (2.37%; 95%CI 1.8-2.8%) out of 3298 nasopharyngeal swabs specimens were found to be positive for OC43 (36;1.09%), HKU1 (34; 1.03%), NL63 (6; 0.18%) and 229E (2;0.01%). None of SARS or MERS was detected. The HCoVs predominant circulating season was in transition of winter to spring, especially January and February and NL63 detected only in summer and fall. Complex population with an abundant genetic diversity of coronaviruses was circulating and they shared homology with the published strains (99-100%). Besides, phylogenetic evolutionary analysis indicated that OC43 coronaviruses were clustered into three clades (B,D,E), HKU1 clustered into two clades(A,B) and NL63 clustered into two clades(A,B). Moreover, several novel mutations including nucleotides substitution and the insertion of spike of the glycoprotein on the viral surface were discovered.ConclusionsThe detection rate and epidemic trend of coronaviruses were stable and no obvious fluctuations were found. The detected coronaviruses shared a conserved gene sequences in S and RdRp. However, mutants of the epidemic strains were detected, suggesting continuous monitoring of the human coronaviruses is in need among cross-border children, who are more likely to get infected and transmit the viruses across the border easily, in addition to the general public.

Dataset Information

Seroepidemiology of group I human coronaviruses in children.

Publications

Seroepidemiology of group I human coronaviruses in children.

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