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Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation.

ABSTRACT: The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.

SUBMITTER: Brittle AL

PROVIDER: S-EPMC2131725 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation.

Brittle Amy L AL Nanba Yasuaki Y Ito Takashi T Ohkura Hiroyuki H

Experimental cell research 20070525 13

The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A ...[more]

PMID: 17586492

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