Mitosis-specific phosphorylation of Mis18? by Aurora B kinase enhances kinetochore recruitment of polo-like kinase 1.

ABSTRACT: Mis18?, a component of Mis18 complex comprising of Mis18?, Mis18?, and M18BP1, is known to localize at the centromere from late telophase to early G1 phase and plays a priming role in CENP-A deposition. Although its role in CENP-A deposition is well established, the other function of Mis18? remains unknown. Here, we elucidate a new function of Mis18? that is critical for the proper progression of cell cycle independent of its role in CENP-A deposition. We find that Aurora B kinase phosphorylates Mis18? during mitosis not affecting neither centromere localization of Mis18 complex nor centromere loading of CENP-A. However, the replacement of endogenous Mis18? by phosphorylation-defective mutant causes mitotic defects including micronuclei formation, chromosome misalignment, and chromosomal bridges. Together, our data demonstrate that Aurora B kinase-mediated mitotic phosphorylation of Mis18? is a crucial event for faithful cell cycle progression through the enhanced recruitment of polo-like kinase 1 to the kinetochore.

SUBMITTER: Lee M 

PROVIDER: S-EPMC5788582 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature